AraPerox

Peroxisome Biology of Plants and Microalgae

Research group by Prof. Dr. S. Reumann
Centre for Organelle Research (CORE)
University of Stavanger, Norway		 UiS logo

Microalgae

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 		Reumann lab > Group members 

Amit Kumar Sharma
Amit_1
Amit Sharma


Algae plate
Plate with microalgae


Amit_2
Cloning of Nannochloropsis genes
Position M. Sc.  student
Nationality Nepalese
M. Sc. project Establishment of synthetic biology for the oil-producing microalga Nannochloropsis
Microalgae attract increasing biotechnological interest due to their high biomass productivity including oils and omega-3 fatty acids. These are very important ingredients as fish feed to Norwegian aquaculture. The major objective of this M. Sc. project is to establish synthetic biology tools for the oil-producing microalga Nannochloropsis. Fundamental molecular technology shall established, including exon cloning from genomic DNA, full-length cDNA cloning by RT-PCR, cloning of constitutive and inducible promoters and UTRs, nuclear transformation, cDNA overexpression and gene knock-out technology by homologous recombination. In the long-run, these methods shall be applied to optimize metabolic pathway optimization and increase the oil and PUFA levels in microalgae. 
Education
2012-present
 Master’s degree in Biological Chemistry (University of Stavanger, Norway), tentative thesis title: “ Establishment of synthetic biology for the oil-producing microalga Nannochloropsis”; supervisor: Prof. Dr. Sigrun Reumann
2004-2009
 Bachelor of Technology (BTech), Biotechnology, Kathmandu University, Nepal; thesis title: "Production and Optimization of ethanol from cheese when using Saccharomyces cerevesiae and Kluveromyces lactis (NCYC752)"
     
Publications
 Pandey K.R., Maden N., Poudel B., Pradhananga S., and Sharma A.K. (2012) The Curation of Genetic Variants: Difficulties and Possible Solutions. Genomics, Proteomics & Bioinformatics 10, 317-325
Lamichhane J., Karki T., Nepal A., Budathoki M., and Sharma A.K. (2012) Production and optimization of ethanol from cheese whey by constitutive use of Saccharomyces cerevisiae and Kluyveromyces lactis NCYC752. J. Biomolecule Reconstruction 9: 1-7
Research interests

Research expertise		 Bioinformatics, molecular biology, algae

DNA Gel Electrophoresis, Bacterial Culture, Transformation, Molecular Cloning, Microscopy, plant tissue culture
Contact Amit Kumar Sharma
Centre for Organelle Research (CORE)
University of Stavanger
Richard Johnsonsgate 4
N-4021 Stavanger, Norway
Phone:  +47-51831817 (Laboratory)
Email: ak.sharma[at]stud.uis.no
Homepage: http://core.uis.no; www.ux.uis.no/reumann